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  • rnaeye
    replied
    Why do not you try enzymatic shearing instead. You can use plate format:
    NEBNext® dsDNA Fragmentase®
    This is the default text for fallback 404 continue text

    Leave a comment:


  • Silvia Weidner
    replied
    Problems in shearing

    Hi erverybody,
    i am new in this field. We have a pgm and i started the gDNA shearing.
    I have big problems to get the right size.
    Can you give me some tips how you optimize your shearing? On which aperatur do you check your sizes? Is it mormal to see only smear?

    Thanks a lot
    Greetings from Munich

    Silvia

    Leave a comment:


  • Profile Genomics
    replied
    Hi Minyong,

    We tried sending you an email yesterday through SEQanswers with some rough estimate info and contact details -- if you don't use your main email for here please check the one you use. Looking forward to talking with you about your project.

    Best,
    Profile Genomics

    Leave a comment:


  • minyongis
    replied
    Request for Quote?

    Hi Profile Genomics,

    I was wondering how much you would charge for the shearing of about 1000 samples and what the turnaround time would be. A rough estimate would be fine...


    Thanks,

    Minyong

    Originally posted by Profile Genomics View Post
    Profile Genomics is part of the Peralta Foundation and is launching a new Genomics facility in Alameda California. One of our premier services is DNA shearing on the Covaris LE220R; we were the first to receive this instrumentation model. Currently we are starting to cut DNA for others. We can do short cuts in 96 well plates, as well as long cuts of 1.5kb – 5kb.

    We look forward to talking with anyone who is interested in this service.

    Leave a comment:


  • simonandrews
    replied
    We're in the process of purchasing a Covaris and will almost certainly take in external samples once it's set up (some time in the next month or so) as part of our sequencing service. Contact me directly ([email protected]) if you might be interested.

    Leave a comment:


  • klytie
    replied
    Thank you!
    I have already contacted KBiosciences in UK (sorry for not mentioning this before) but this was like two weeks ago and no answer so far. So I was wondering whether there was some other place providing this service.

    Leave a comment:


  • Profile Genomics
    replied
    Covaris in Europe

    Hi - Covaris' European dealer is KBioscience, which was recently acquired by LGC. Here is their website with their contact info at the bottom of the page...


    ...and here is their Covaris page:


    In the US you can obtain shearing services directly from Covaris, they are higher priced than other providers but obviously intensely knowledgeable about capabilities and protocols; we're not familiar with the entire situation in Europe but you might check into KBioscience to see what services and advice they can offer you in your area.

    Leave a comment:


  • klytie
    replied
    Hi! Does anyone know is there Covaris shearing service provider somewhere in Europe also?
    Would be very-very thankful for the information.

    Leave a comment:


  • Profile Genomics
    replied
    DNA shearing service

    Profile Genomics is part of the Peralta Foundation and is launching a new Genomics facility in Alameda California. One of our premier services is DNA shearing on the Covaris LE220R; we were the first to receive this instrumentation model. Currently we are starting to cut DNA for others. We can do short cuts in 96 well plates, as well as long cuts of 1.5kb – 5kb.

    We look forward to talking with anyone who is interested in this service.

    Leave a comment:


  • sunsnow
    replied
    what is you budget per sample? we could do it

    Leave a comment:


  • delbar
    replied
    Hi all- I have not had success in outsourcing the DNA shearing- we got quotes from Covaris and Expression Analysis (here in NC), but the $ was out of reach for what we can do right now.

    We need to have the samples sheared to 150-200 bp and will be using the Agilent SureSelect Library Prep and Capture System.

    Leave a comment:


  • Hamid
    replied
    Hi Delbar,

    What size range fragments are you interested in getting?
    Last edited by Hamid; 11-16-2011, 10:14 PM.

    Leave a comment:


  • Lisamae19
    replied
    delbar,
    Were you successfull in outsourcing your DNA shearing?

    Leave a comment:


  • jo_mason
    replied
    Originally posted by delbar View Post
    Hello- I work for a lab at UNC-Chapel Hill and we are hoping to find a way to outsource shearing of at least 1000 DNA samples. We need to keep these in 96 well plate format, and would be doing this in batches of 3-4 plates at a time, therefore we are searching for a company or academic core facility with an instrument capable of high-throughput shearing. We have already contacted Covaris, but the cost was way outside of our budget.
    Thank you for any suggestions!
    We could do this for you at MGRC, but it would mean you shipping samples all the way to Malaysia and back, and that will bump up your costs. If you are desperate get in touch: [email protected] but there surely must be someone closer to you with a Covaris set up to do this.

    Leave a comment:


  • adaptivegenome
    replied
    Would a Bioruptor or hydroshear work? Sorry in advance if it is a naive suggestion.
    Last edited by adaptivegenome; 11-09-2011, 05:43 PM. Reason: typo

    Leave a comment:

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