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  • Anyone can refer good and not too expensive exome sequencing facility?

    We are looking at a small pilot project, 50-100 exomes (we prefer Illumina based core). Already have some exp with OtoGenetics but their SureSelect capture based option seems pricey. Anyone ? Thanks (not sure if that is the right category to ask for it but....)
    Last edited by memento; 02-15-2012, 09:54 AM.

  • #2
    Originally posted by memento View Post
    We are looking at a small pilot project, 50-100 exomes (we prefer Illumina based core). Already have some exp with OtoGenetics but their SureSelect capture based option seems pricey. Anyone ? Thanks (not sure if that is the right category to ask for it but....)
    A leading provider of clinical research and diagnostic solutions. We work in partnership with customers to progress the future of genetic-based medicine.


    Obvious disclaimer applies!

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    • #3
      BGI,you may choose.

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      • #4
        We've tested a number of exome service providers and are now going to regularly use Axeq.
        We were quoted <£700 per exome using Nimblegen's latest capture.
        We had a number of communication problems and delays with BGI.

        Comment


        • #5
          Originally posted by TonyBrooks View Post
          We had a number of communication problems and delays with BGI.
          What was the matter...wasn't Ryan getting back to you in time?

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          • #6
            $999 exome

            We do $999 at the Columbia Genome Center. That includes all reagents, Agilent SureSelect XT Human All Exon v.2 44Mb, Library preparation, 60X depth mapped coverage, 100bp Paired End reads, sequenced on Illumina HiSeq2000 instruments, 2 months data storage, QC, reads mapped to the human reference genome, single nucleotide variants and short insertions/deletions, basic functional annotation of the variants, and summary statistics. Our average turnaround was 55 days last quarter.

            I can send you recommendations from PIs who use our service. We take orders from all over the world.

            We will be happy to answer any questions at [email protected]

            Our FAQ is here:
            The Columbia Genome Center is a specialized environment for conducting high-throughput biomedical research. Our services include: Genome Sequencing & Analysis  High-Throughput Screening 
            Last edited by ocouronne; 02-21-2012, 10:58 AM.

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            • #7
              Originally posted by TonyBrooks View Post
              We've tested a number of exome service providers and are now going to regularly use Axeq.
              We were quoted <£700 per exome using Nimblegen's latest capture.
              We had a number of communication problems and delays with BGI.
              so can you detail the problems?maybe we can give you feedback.

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              • #8
                Originally posted by Gone View Post
                so can you detail the problems?maybe we can give you feedback.
                If he is in the UK, shouldn't your European subsidiary be addressing these issues? Or do they lack the competence to do so?

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                • #9
                  Originally posted by Geneus View Post
                  If he is in the UK, shouldn't your European subsidiary be addressing these issues? Or do they lack the competence to do so?
                  Yeah,U're right!BGI-Europe will handle all issues in local region.
                  Anyway,we'll try our best to do any kinds of problems.
                  you know customer forever is God!

                  Comment


                  • #10
                    Originally posted by Gone View Post
                    Yeah,U're right!BGI-Europe will handle all issues in local region.
                    Anyway,we'll try our best to do any kinds of problems.
                    you know customer forever is God!
                    Yes...that's the way Mr. Mason would want it.

                    Comment


                    • #11
                      Best and smartest sequencing facility around is Expression analysis

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                      • #12
                        Originally posted by kamichailo View Post
                        Best and smartest sequencing facility around is Expression analysis
                        This should (perhaps obviously) be noted as biased.

                        Comment


                        • #13
                          Originally posted by ECO View Post
                          This should (perhaps obviously) be noted as biased.
                          LOL...nothing like full disclosure.

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