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  • Illumina samplesheet fields

    Hi there,

    A samplesheet (for demultiplexer.pl) as the following headers:
    FCID,Lane,SampleID,SampleRef,Index,Description,Control,Recipe,Operator

    What is "Recipe" meaning ?

    Thanks
    Last edited by nicolallias; 08-18-2010, 07:24 AM.

  • #2
    When the techs start the sequencer they load a recipe - it's what tells the instrument how many cycles to run, etc. I don't believe it's required for the demultiplex.pl script. It's info that would have been generated if you used the cluster station/cBot to create the sample sheet.
    Christine Brennan
    UM DNA Sequencing Core
    Ann Arbor, MI 48109

    [email protected]

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    • #3
      Thanks for your reply

      Comment


      • #4
        Another difference – a fresh one – additionally comes into play whilst we remember fractional powers. Suppose that the fractional strength m/n has been decreased to lowest phrases (all commonplace place elements within side the numerator and therefore the denominator were cancelled). To calculate xm/n, we continue in steps: 1) stilleducation Find the n th root of x (x1/n = ); 2) Raise it to the m th strength. The 2d step is straightforward: we will boost any big variety to an integer strength. the primary step, however, is complex for poor x : We can’t meaningfully invite a good root of a poor big variety . Thus, the allowable inputs (domains) of fractional powers depend on whether or not n is even or bizarre.

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