Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • RNA-Seq Sample Question

    Hi all,

    I am preparing samples to send for RNA-sequencing. I've transformed a majority of my samples to cDNA, keeping only a few microliters of highly concentrated RNA for QC analysis, as I had concerns over the RNA stability. However, on the sequencing service page, it seems as if most samples are sent in RNA form. Has anyone successfully sent cDNA samples for RNA-sequencing?

    Thanks in advance,
    Michael

  • #2
    The commercial RNA-Seq kits are using RNA as input, not cDNA. You have converted RNA to cDNA, the single-stranded DNA? It may be a problem for the sequencing cores.

    Comment


    • #3
      Of course you can submit cDNA for sequencing, at this point it's just 'DNA'. Most cores would probably prefer to take the RNA because then the library preparation is in their hands, and not yours - and they can do the QC that follows the process to maximise their confidence in the seqeuncing of your samples.

      You just need to speak to the provider and tell them what the DNA source is.

      Next time, just send the RNA.

      Comment


      • #4
        Bukowski is correct. RNA-seq library prep protocols (starting from RNA) are optimized for consistency to allow quantitative DGE analysis. Your cDNA prep protocol might not be.
        Is your cDNA double-stranded (as required for most DNA library preps) or single-stranded?

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Genetic Variation in Immunogenetics and Antibody Diversity
          by seqadmin



          The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
          11-06-2024, 07:24 PM
        • seqadmin
          Choosing Between NGS and qPCR
          by seqadmin



          Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
          10-18-2024, 07:11 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 11-08-2024, 11:09 AM
        0 responses
        59 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 11-08-2024, 06:13 AM
        0 responses
        38 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 11-01-2024, 06:09 AM
        0 responses
        35 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 10-30-2024, 05:31 AM
        0 responses
        23 views
        0 likes
        Last Post seqadmin  
        Working...
        X