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SEQanswers June Challenge Has Begun!

The competition has begun! We're giving away a $50 Amazon gift card to the member who answers the most questions on our site during the month. We want to encourage our community members to share their knowledge and help each other out by answering questions related to sequencing technologies, genomics, and bioinformatics. The competition is open to all members of the site, and the winner will be announced at the beginning of July. Best of luck!

For a list of the official rules, visit (https://www.seqanswers.com/forum/sit...wledge-and-win)
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  • begsch
    replied
    --> http://seqanswers.com/forums/showthread.php?t=4819

    Originally posted by AKroxy View Post
    Here is the info I got from the Roche rep when I asked for the RAPID adaptor sequences so I could design PCR primers.

    The adaptors are double stranded. The sequences TCAG at 3' end of each long adaptor strand are the general Ti shotgun key sequences. The key sequence is different with Rapid Library. The following figure will help in explaining better. You can order the Primers below to check your library.

    emPCR Primer A 5' CCATCTCATCCCTGCGTGTC 3'
    TI A Adaptor 5' CCATCTCATCCCTGCGTGTCTCCGACTCAG 3'
    3' AGAGGCTGAGTC 5'

    emPCR Primer B 5' CCTATCCCCTGTGTGCCTTG 3'
    TI B Adaptor 5' CCTATCCCCTGTGTGCCTTGGCAGTCTCAG 3'
    3' ACCGTCAGAGTC 5'

    Leave a comment:


  • qkdlspf
    replied
    I'd also like to know the adaptor sequences.

    Leave a comment:


  • SeqTruth
    started a topic Adapter sequences

    Adapter sequences

    I'm looking for the sequences of both the 454 FLX and the Titanium library adapters. I'd also be interested to know the adapter sequences of any of the other platforms.

    Thanks!

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