rociogma that's so strange. I'm assuming the libraries looked fine on the Qiaxcel Advanced?
One issue can be during the pooling and denaturation. I'm not suggesting that you made a mistake, but if the Tris-HCl or NaOH went bad or was at the wrong concentration then the libraries won't bind to the flow cell. If you can maybe check the pH of your NaOH and Tris-HCl.
My main suggestion is to check the flow cell and write down the barcode number. I think you should take a picture of it for your records. I would then contact Illumina and double check there wasn't anything complaints with that batch of flow cell. Illumina always helps with stuff like this and their support staff is pretty good.
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I have prepared the libraries with Qiaseq kit and Qubit4, and the quality was checked by Qiaxcel Advanced.
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sequence456789 and rociogma before you loaded your libraries, did you check them on a Bioanalyzer or Tapestation? I'm curious if there tons of adapter dimers.
What was the library prep kit used? And how did you quantify them? There's always the possibility of accidentally making a mistake during the pooling, denaturation, or loading steps.Leave a comment:
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No data?? Any advice
Sequencing on the Illumina MiSeq cluster generation complete - but no clusters passing filter or Q30 or data generated.
image here : https://postimg.cc/Wh3xpzCt
I know it is over clustered but where are the (even though it is probably crappy) FastQ files? It would be nice to have a look anyway.
The run did not terminate and completed all cycles with “no errors”.
Any advice besides reloading the run after redoing quantification?
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The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i
...01-24-2023, 01:19 PM -
Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.
The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes...01-09-2023, 03:10 PM
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