Nope you are unlikely to sequence "all" your fragments since you sequence only a fraction of the library that you load on the flowcell.
You always sequence fragment)s). If you stop after sequencing one of the ends then that is all you get. If you happen to sequence from the other end they you get spatial information that tells you how long the original library fragment is. You can know this in two ways. If you have a reference genome you can map your reads to that genome and figure out how far apart they map. If you don't have a reference genome but your insert sizes are small then you can merge the two reads to create a longer representation that gives you the size if the insert. If you only start with 150 bp inserts and say do 2 x 100 bp paired end sequencing then your reads will overlap in the middle.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Yes I'll sequence 400M molecules. But if my fragmented DNA is around 150 bp the difference compared single and paired is that in the latter I'll sequence all my fragments, isn't it?
Leave a comment:
-
If you count like Illumina does, then yes these would be 800M reads. You will still sequence only 400M library molecules though.
Leave a comment:
-
reads number in single-end vs paired end seq
Hi all,
probably a stupid question but, i'm going to use NextSeq500 and preparing my sample with TruSeq Nano library prep kit.
Sequencing will be performed by the V2 kit, 75 cycles.
So, the stupid question is:
the reads count with single end seq is up to 400M, does it means that the paired end seq will produce up to 800M reads??
Many thanksTags: None
Latest Articles
Collapse
-
by seqadmin
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...-
Channel: Articles
10-07-2024, 08:07 AM -
-
by seqadmin
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...-
Channel: Articles
09-23-2024, 06:35 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 10-02-2024, 04:51 AM
|
0 responses
104 views
0 likes
|
Last Post
by seqadmin
10-02-2024, 04:51 AM
|
||
Started by seqadmin, 10-01-2024, 07:10 AM
|
0 responses
112 views
0 likes
|
Last Post
by seqadmin
10-01-2024, 07:10 AM
|
||
Started by seqadmin, 09-30-2024, 08:33 AM
|
1 response
116 views
0 likes
|
Last Post
by EmiTom
10-07-2024, 06:46 AM
|
||
Started by seqadmin, 09-26-2024, 12:57 PM
|
0 responses
22 views
0 likes
|
Last Post
by seqadmin
09-26-2024, 12:57 PM
|
Leave a comment: