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Update: Talked with Qiagen. The problem seems to be that Qiazol is not compatible with the gEliminator column, because of the acidity. -
28s Missing After gEliminator
I have been extracting RNA from heart tissue for almost 10 years. I usually use the Qiagen RNeasy kit with on column DNAse digestion. This month I tried the RNeasy Plus kit that comes with gEliminator tubes (to remove genomic DNA contamination). When I ran a bioanalyzer to confirm quality I noticed that all the samples I extracted with the gEliminator tubes are missing the 28s peak. Has anyone else ever noticed this?
With gEliminator step
With RNAse free DNAse on column digestion
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