Hi,

I have a question about multiplication factor for library quantification after KAPA qPCR. We have been advised to multiply the qPCR result by the factor of standard fragment size (452bp) divided by the library size (~230bp). For this case it's a factor of 2.

This means our qPCR results are twice as concentrated as our qubit and bioanalyser results. It doesn't sound right but qPCR has always ben accurate for us. Is the multiplication factor wrong? We haven't seen this before because our libraries are usually much closer to the standard fragment size of 452bp.

I'm looking on the KAPA website but thought I would also post here.

I have a question about multiplication factor for library quantification after KAPA qPCR. We have been advised to multiply the qPCR result by the factor of standard fragment size (452bp) divided by the library size (~230bp). For this case it's a factor of 2.

This means our qPCR results are twice as concentrated as our qubit and bioanalyser results. It doesn't sound right but qPCR has always ben accurate for us. Is the multiplication factor wrong? We haven't seen this before because our libraries are usually much closer to the standard fragment size of 452bp.

I'm looking on the KAPA website but thought I would also post here.

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