Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Hello! new on NGS

    Hi all!

    I am planning to request a RNA-Seq service from a local provider, but I am not clear about the following things:

    If I just want to compare the global gene expression of two CHO cell samples, are 50 bp single sequence reads good enough for the analysis? or paired end reads are required?

    In the GAIIx system, is one lane enough to run two different CHO mRNA-libraries, considering that the CHO genome contains around 24,000 genes?

    Which system is better GAIIx or HiSeq2000?

    Thank you, hope someone with experience on this can give me some advice!

    Verenice.

  • #2
    The answers to these questions probably depend on how much money you are prepared to spend -- "good enough" is different for different people. If you want the best results, use paired-end sequencing with as many reads as possible.

    Comment


    • #3
      Thank you gringer!

      You are right, first of all I need to consider my budget for this analysis.

      Have a nice day!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Exploring the Dynamics of the Tumor Microenvironment
        by seqadmin




        The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
        07-08-2024, 03:19 PM
      • seqadmin
        Exploring Human Diversity Through Large-Scale Omics
        by seqadmin


        In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...
        06-25-2024, 06:43 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 05:49 AM
      0 responses
      15 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 07-15-2024, 06:53 AM
      0 responses
      26 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 07-10-2024, 07:30 AM
      0 responses
      37 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 07-03-2024, 09:45 AM
      0 responses
      204 views
      0 likes
      Last Post seqadmin  
      Working...
      X