Hello, my name is Ksenia. I am writing a master's thesis. To do this, I need to understand what methods of probe synthesis companies use, such as Agilent/IDT, etc. I know classical synthesis methods consisting of 4 stages, some types of printing, PCR synthesis and synthesis using ligase (chemical-enzymatic). But for example, Agilent receives probes based on SurePrint technological equipment operating on the principle of ink-jet printing. What does the synthesis scheme look like in this case? I have assumptions that some stages of classical organic synthesis are absent in this case, because they are unnecessary and in order to increase the speed and yield of synthesis.
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