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  • pbluescript
    replied
    The two main programs people use for SNP detection are samtools and GATK. I'd start with reading the manuals for those.

    Leave a comment:


  • waseem
    replied
    Thank you very much.
    It helped a lot.
    Next I wanted to do is "to identify polymorphisms in arabidopsis genome" and I have paired end DNA sequences. any hint about where to start?

    Leave a comment:


  • ttnguyen
    replied
    There is a tutorial for the analysis of RNA-seq data:
    Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

    Leave a comment:


  • pbluescript
    replied
    Watch some tutorial videos at Galaxy:
    Galaxy is a community-driven web-based analysis platform for life science research.

    Or, if you're comfortable with the command line, jump right in with something like Tophat.

    Leave a comment:


  • nirvana
    replied
    Maybe you could begin with trying some align softwares, such as BWA

    Leave a comment:


  • waseem
    started a topic New to RNAseq

    New to RNAseq

    Hello
    I am new to RNAseq analysis.
    Jut got my sequences and I want to start.
    I want to allign the reads to reference transcriptome.
    Any guidelines or help for a beginner.

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  • seqadmin
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