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A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology.

Biotechniques. 2011 Sep;51(3):195-7

Authors: Morozumi T, Toki D, Eguchi-Ogawa T, Uenishi H

Abstract
Large-scale cDNA-sequencing projects require an efficient strategy for mass sequencing. Here we describe a method for sequencing pooled cDNA clones using a combination of transposon insertion and Gateway technology. Our method reduces the number of shotgun clones that are unsuitable for reconstruction of cDNA sequences, and has the advantage of reducing the total costs of the sequencing project.


PMID: 21906043 [PubMed - indexed for MEDLINE]



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