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This is an amusing post to look back on three years later. It would have been even more interesting if people had posted their predictions.
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Which name henceforth ?
Ok, I am genuinely curious to know what people think of the three acronyms and which one will last.... I must say that while I've heard a multitude of people use the term RNA-seq at conferences and within ENCODE, I had not heard the terms WTSS and SQRL until I looked at the papers.
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SQRL is on the front page, but it's a good thread to start. There are many important papers and methods coming out now.
You forgot another very important one...
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WTSS, SQRL or RNA-seq
In the last couple of weeks, 4 papers have come out that all describe the sequencing of mammalian transcriptomes with short reads. It has been termed, by different groups, Whole Transcriptome Shotgun Sequencing, Short Quantitative Random RNA Libraries, and RNA-seq. I haven't seen any posts here on it, so I thought I would provide the links:
(application to mammals)
mouse:
human:
An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organisms
(yeast papers)
Recent data from several organisms indicate that the transcribed portions of genomes are larger and more complex than expected, and that many functional properties of transcripts are based not on coding sequences but on regulatory sequences in untranslated regions or non-coding RNAs. Alternative sta …
The identification of untranslated regions, introns, and coding regions within an organism remains challenging. We developed a quantitative sequencing-based method called RNA-Seq for mapping transcribed regions, in which complementary DNA fragments are subjected to high-throughput sequencing and map …Tags: None
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Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...-
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