We have started a formal thread for current and future users to discuss DNA sequins:
or RNA sequins:
Enjoy!
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Hi nucacidhunter,
DNA sequins can be used with any sequencing platform. An application where the combination of long-read sequencing (eg PacBio) and Sequins will be particularly powerful is in the detection of structural variation (which we all know is a major challenge). Our current generation of Sequins includes longer standards that emulate large deletions, inversions, tandem duplications, novel sequence insertions and mobile element insertions, all based on real instances of human variation.
Cheers
Ira Deveson (on behalf of the Sequins team)
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It will be good if they can design longer DNA Sequins for PacBio or 10x Genomics Chromium platform.
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Spike-in controls
Spike-in controls is one of the oft discussed topics on SeqAnswers.
Researchers at the Garvan Institute of Medical Research have just published two papers in Narure Methods about synthetic DNA and RNA standards that can be used as spike-in controls.
Deveson et al: http://www.nature.com/nmeth/journal/...meth.3957.html
DNA Controls
Sequencing reads derived from sequins align exclusively to an artificial in silico reference chromosome, rather than the human reference genome, which allows them them to be partitioned for parallel analysis.
RNA Controls
Sequins have an entirely artificial sequence with no homology to natural reference genomes, but they align to gene loci encoded on an artificial in silico chromosome. The combination of multiple sequins across a range of concentrations emulates alternative splicing and differential gene expression, and it provides scaling factors for normalization between samples.
Latest Articles
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by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
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