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  • Technology Applications Highlighted at Nanopore Community Meeting

    Oxford Nanopore Technologies (ONT) concluded its community meeting in New York City. The event, which took place December 5 through the 7, highlighted nanopore's real-world applications and emphasized the importance of their sequencing technology.

    Brief courses and technical exhibits were also incorporated into the meeting, including an onsite loading of a flow cell and loading demonstrations with VolTRAX, ONT’s automated library prep device. Sessions on data analysis were conducted by the company’s application scientists, which focused on basecalling, methylation detection, variant calling, and generating assemblies.

    Researchers from all over the world participated in the event and described the importance of ONT devices in their work. Notable talks from scientists included one by Karen Miga, Ph.D., from the University of California Santa Cruz, who spoke about her collaborative efforts that utilized ONT devices to complete the human genome, an achievement that was recently published in Science

    Other presentations from researchers described the application of long-read sequencing to identify SNPs, DNA methylation, and variant detection related to disease, tasks that were increasingly difficult using short-read technologies.

    The meeting also involved a technology update from several of ONT’s scientists. These updates include:

    • Expected release of R10.4.1 flow cells in combination with Kit 14 for high-yield and high-accuracy sequencing in the first quarter of 2023.
    • Updates to chemistry, new enzymes, and basecalling developments allowing for increased sequencing speeds, higher data outputs, and characterization of diverse base modifications.
    • New RNA-specific nanopores for increased accuracy and lower inputs for direct RNA sequencing.
    • Progress of a method to sequence DNA and RNA directly from biological fluids without assistance from equipment.
    • Development of a method, Outy, that reverses direction of sequencing through a nanopore, allowing for single molecule re-reads (forward and backward through a nanopore).

    The next big nanopore event, London Calling 2023, will take place May 17–19 and includes an onsite and a virtual component. Find out more about the Nanopore Community Meeting here.

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