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  • YTree 1200 Sardinian samples from Paolo Francalacci's article

    This is some very exciting news that I was lucky enough to be involved in a small way. Many new samples from Paolo Francalacci's paper are being added to the YFull tree. More information and comments can be seen at the Yfull Facebook page. I would like to thank Paolo and Vadim for their efforts in this endeavor. I hope this is the beginning of academic data being brought into a public forum for all to see. Current Technical requirements: .FASTQ or .BAM file; coverage min 25X; read length min 100 bp for determining age estimations. Currently the YTree at YFull is at revision 3.17.

    Vadim Urasin YFull Principle
    17 hrs ยท Edited
    "We started to add in our YTree 1200 Sardinian samples from Paolo Francalacci's article http://www.ncbi.nlm.nih.gov/pubmed/23908240 Kerry O'Dair thank you for help with first communication with Paolo. At first we will add 49 samples. They have ids 4399-4401, 4411, 4416, 4592-4632 (numbers of 3 samples indefined yet). Later we create special page with list of samples anf their subclades and we will change way to show them in our YTree and add reference to Paolo's article.
    Unfortunately we can not add these samples in any group for reason of keeping privacy. Also we will not use these sample for age estimation because of their low quality. But you will see changes in our YTree. I think we will define many new subclades. Each batch we plan to add about 60 Sardinian samples.
    We don't promise to add all of 1200 samples but we will add many of them."

    Breakdown of subclades in study:
    http://imgur.com/pKVZW93
    Last edited by KerryOdair; 11-22-2015, 05:44 AM. Reason: Add subclades in study
    FullGenomes Kit 045DV YFull Terminal SNP Y2846 FTDNA Kit 52277 M35>V12>CTS693>CTS3346>Y2877>CTS6667>CTS8411>Y2846 MTdna U4b1a3a

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    • Whole Genome Sequencing and Y Chromosome Sequencing

      There has been an interesting discussion at Rootsweb about Whole Genome Sequencing and Y sequencing. The entire thread can be read there.

      http://archiver.rootsweb.ancestry.co...GY-DNA/2016-01

      I have distilled some of the conversation into three points of information coming from Justin Loe Fullgenomes, Thomas Krahn and Tim Janzen. All three of these folks have been leaders in the area of testing over the years. We are coming eventually to a price point where Whole Genome Sequencing will probably replace sequencing the Y. The conversations below will make this more clear.

      FullGenomes
      Whole Genome Sequencing 2x $280
      Whole Genome Sequencing 4x $395
      Whole Genome Sequencing 10x $725
      Whole Genome Sequencing 15x $895
      Whole Genome Sequencing 20x $1,200
      Whole Genome Sequencing 30x $1,650
      Ultimate 100x Whole Genome $5,350
      Y Elite 2.0 Sequencing $775


      The largest cost factor for Y chromosome tests is not the sequencing, but the enrichment procedure. With enrichment the price will never drop far below $400, so you might as well sequence with sufficient coverage.

      On the long run there is no future for BigY and FGCs Y tests since the WGS tests keep dropping in the price, but Y tests don't.

      Thomas Krahn

      Justin Loe from FullGenomes just posted the information below on another list:


      Mapped Y coverage
      30x 22,856,938
      10x 22,025,697
      4x 17,678,170
      2x 13,755,442

      Average Callable Loci
      30x 14,558,001
      10x 8,046,540
      4x 1,050,996
      2x 349,397

      Y Elite 2.0:
      14,000,000 Callable Loci approximately on average"

      It thus appears that one needs to order at least the 30x whole genome sequence from FullGenomes to get the same number of callable Y chromosome loci as you can get from the Y Elite 2.0 test.

      I would like to point out that even Full Genomes' Ultimate 100x Whole Genome is not the Holy Grail in whole genome sequencing because the data is unphased. What we are still waiting for is a company to generate phased whole genome sequencing data with an extremely high level of accuracy. I know that companies are working on this, but so far it has been challenging for the companies to do.

      Sincerely,
      Tim Janzen
      Last edited by KerryOdair; 01-10-2016, 07:50 AM.
      FullGenomes Kit 045DV YFull Terminal SNP Y2846 FTDNA Kit 52277 M35>V12>CTS693>CTS3346>Y2877>CTS6667>CTS8411>Y2846 MTdna U4b1a3a

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      • This is a very nice announcement and shows how far we have come in products available to the public.

        PUBLIC RELEASE: 28-JAN-2016
        Full GenomesTM Corporation collaborates with Novogene to offer low-cost whole genome ancestry test
        Comprehensive GenomeGuide now available at $895

        It will be in the range of 15x - 20x, since our results that is the range that gives good Y chromosome coverage. 20x is equivalent to Y Elite, in terms of the Callable Loci metric. So, its not fixed at specific number but we'll target it in that range as we get more data.

        From other beta data:

        Callable loci
        20x: 13,888,138

        Mapped Y coverage
        20x: 22,818,005

        The various Y Elite tests all average approx 14 million Callable Loci, however, the purpose here isn't to replace the Y Elite product, although ultimately, it is possible that a whole genome test will be no more expensive.
        --
        Justin Loe, M.S. Bioinformatics

        NOVOGENE CORPORATION

        SHARE PRINT E-MAIL
        Full GenomesTM Corporation, the first company to offer a high-resolution and comprehensive Y chromosome test in January 2013, announced today that it is collaborating with Novogene, a leading genomics solution provider with the largest Illumina-based sequencing capacity in China, to offer GenomeGuide , one of the first whole genome tests for ancestry purposes for under $1,000.

        GenomeGuide, now available to consumers at $895, includes raw data (BAM file), variant summary reports from SnpEff and VEP that are compatible with third party tools, such as Promethease, autosomal and X-chromosomal variant identification (Variant Call Format) files , and mitochondrial and Y chromosome reports (for males). As with other Full Genomes products, GenomeGuide is intended for ancestry/research-use only, and should not be relied upon for medical or diagnostic purposes.

        Novogene, the only Illumina Genome Network partner in China, will deliver high-quality WGS data using the Illumina Hi-Seq X Ten system capable of sequencing up to 18,000 human genomes per year at the lowest cost per genome, and will apply its advanced bioinformatics capabilities and expertise to provide variant analysis.

        "The advent of new technology has enabled Full Genomes to offer GenomeGuide, a new whole genome ancestry test which will be the most comprehensive ancestry test on the market today," stated Justin Loe, CEO of Full Genomes. "Full Genomes is committed to providing responsible and detailed genetic reports to the customer," he added, "and we are incorporating the latest technology to enable the consumer to receive comprehensive information on their ancestry. With their advanced Illumina technology, outstanding informatics/analysis, and highly responsive and effective support, we are confident that Novogene will deliver the high-quality WGS results our customers expect."

        "We look forward to collaborating with Full Genomes and to helping enable the delivery of this highly cost competitive ancestry research to consumers," stated Dr. Ruiqiang Li, Founder and Chief Executive Officer of Novogene. "As one of the first companies in the world to purchase Illumina's HiSeq X Ten in early 2014, we have extensive experience with the system and are uniquely positioned to provide Full Genomes and its customers with the highest quality WGS data."

        ###

        About Full Genomes Corporation

        Full Genomes incorporated in 2012 for the purpose of making full genomic sequencing for genealogical use available to the general public at a leading price point. Full Genomes introduced Y Elite, a comprehensive (next generation sequencing) of the Y chromosome in 2013 to the genetic genealogy market. Since then, a variety of customers in the U.S. and overseas, as well as a number of institutions have used FGC's Y chromosome product. Full Genomes' proprietary DNA analysis capabilities for the Y chromosome have been recognized and have been used for a variety of research projects. Full Genomes has partnered with various vendors and organizations for sequencing and chip development with the goal of advancing new products and DNA services addressing additional markets.

        About Novogene

        Novogene Bioinformatics Technology Company, Ltd., headquartered in Beijing with branches in the US and UK, is a leading genomics solution provider with cutting edge bioinformatics expertise and the largest Illumina-based sequencing capacity in China. Committed to quality service and scientific excellence, Novogene has achieved rapid growth and industry recognition by working in partnership with diverse healthcare, educational and research institutions around the globe to realize the unlimited potential of the rapidly evolving world of genomics. The company has completed numerous major service projects with findings published by top-ranked journals such as Science and Nature. Novogene is the first company in China to purchase Illumina's HiSeq X 10 system and is the only Illumina Genome Network partner in China. Novogene Corporation is Novogene's U.S. subsidiary, based in San Diego, CA. To learn more, visit http://en.novogene.com.
        FullGenomes Kit 045DV YFull Terminal SNP Y2846 FTDNA Kit 52277 M35>V12>CTS693>CTS3346>Y2877>CTS6667>CTS8411>Y2846 MTdna U4b1a3a

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        • New test available for whole genome to general public from FullGenomes as a pilot project.

          New from Justin Loe at FullGenomes

          FGC has a new offering, offered as a pilot project, the long read whole genome, which has 10 Mb haplotype blocks. This is a dramatic improvement on the current short-read whole genome technology of 150 bp. Our offering is currently $2750 per sample.
          https://www.fullgenomes.com/purchases/61/?

          Our offering will also provide advanced ancestry information as well as significant improvements in the data for other applications. We can also refer you to appropriate teams for sophisticated health analysis (FGC does not provide health reports).

          See (technology used)

          http://www.10xgenomics.com/news/10x-...r-information/

          Features from 10xgenomics on full genome testing:

          Solution Features
          Complete genomic information from a single universal library
          Call variants and resolve long-range information from a single library that is compatible with all Illumina sequencers
          Multi-megabase phase blocks
          Greater than 10 Mb haplotype blocks with phased SNVs, indels, and structural variants
          Low DNA input
          High quality libraries from 1 ng of genomic DNA
          Turn-key analysis pipeline and visualization tools
          Powerful algorithms and software for phasing, variant calling and structural variant analysis
          FullGenomes Kit 045DV YFull Terminal SNP Y2846 FTDNA Kit 52277 M35>V12>CTS693>CTS3346>Y2877>CTS6667>CTS8411>Y2846 MTdna U4b1a3a

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          • This is from Vadim Urasin co founder of YFull:

            "We have registered ID YF10000 yesterday. A milestone. Thank you all for orders. By the way, in this batch we have added about a hundred samples from scientific papers of this year (later I will write references to papers). They have a lot of new SNPs. This is a main reason of delay with results."

            This is a remarkable accomplishment in my mind which represents 10,000 samples in the Ytree database. This is a remarkable effort that represents citizen scientists, scientific studies and universities contributing this information in a public forum for everyone to see.

            This I think brings to conclusion this thread and yes we can sequence the Y for all to see. Its been a great ride and it will continue with new data from current and ancient populations. A well done by everyone involved and thanks to SEQanswers that gave me a podium to discuss these issues.
            FullGenomes Kit 045DV YFull Terminal SNP Y2846 FTDNA Kit 52277 M35>V12>CTS693>CTS3346>Y2877>CTS6667>CTS8411>Y2846 MTdna U4b1a3a

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