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  • Want to generate more data to fill in gaps

    Hello,
    This might be a stupid question.
    We are currently working on a ~5M bacterial genome assembly. Our reads data is ~8X coverage, 454 sequencing. The coverage is kind of low. So we used re-guided assembly. Now we found there were still lots of gaps (~500).
    If we want to fill in these gaps, should we resequencing the whole genome on 454? It still costs us too much. Does anybody have any other suggestions?

    Thank you very much.

    Salmon

  • #2
    short read mate-pair may help you to fill the gaps...
    Below link may help you...
    De Novo plant Bac sequencing (http://seqanswers.com/forums/showthread.php?t=1179)

    Comment


    • #3
      Hi Rao,

      Thank you very much for your help.

      Comment

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