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  • #1

    Alternative sequence enrichment methods

    I am looking into alternatives to NimbleGen sequence capture prior to Roche/454 GS FLX sequencing. Does anybody have experience of using the following sequence enrichment methods? Can they be used successfully for Roche/454 GS FLX sequencing?

    Raindance
    http://www.genomeweb.com/sequencing/...hment-platform

    Agilent SureSelect (possibly Illumina only)
    http://www.agilent.com/about/newsroo...b-ca09006.html

    Any other alternatives?
    Tags: None
  • #2
    We are going to test Combimatrix's arrays in the following weeks.
    Have you ever tryed any of the methods you mentioned before? How did they work?

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    • #3
      I checked Combimatrix website, but am not sure how their exon capture works. Could you share some detail?
      --
      bioinfosm

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      • #4
        They do not have a sequence enrichment product (array) out yet. What we are trying to do is testing their arrays to see if they could be usable (and most interestingly for $ , REUSABLE) for seq enrichment. The method of hibridization would be the same as with Nimblegen's or Agilent's arrays.
        Unfortunately the samples are not back yet.. so I ll keep you updated when we have some results from the sequencer

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