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terminology of A/B for emPCR-A kits

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  • terminology of A/B for emPCR-A kits

    Hi 454 users,

    I've never used the emPCR kit for amplicon libraries before and get confused about the terminology of A/B for capture beads, amplification primers, enrichment primers and sequencing primers.

    My hunch is capture beads A should go with amplification primer A, enrichment primer A and sequencing primer A (and same for B) but I would like to confirm with you before I add wrong primers to the capture beads...

    Thanks in advance!

  • #2
    Your hunch is correct. Use all the "A" reagents together and all the "B" reagents together.

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    • #3
      Thanks ajthomas!

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