Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Buying reagents from outside source to replace the 454 DNA library kit

    I heard some people just buy enzymes from outside source instead of buying the DNA library kit from 454. It is much cheaper that way. I would like to try that.
    I was wondering if anyone have a list of where to get the enzymes and protocol for making the library since concentration of the reagents/enzymes might be different.
    Any insight or helpful advice is highly appreciated. Thank you.

  • #2
    NEB already has available a kit to prepare genomic DNA libraries for the Illumina Genome analyzer (http://www.neb.com/nebecomm/products/productE6000.asp) which replaces the Illumina kit at a fraction of the cost. You must supply your own adapters and primers; these can be ordered from Illumina (at great cost) or you can get them synthesized at IDT. We tested the NEB kit, using leftover adapters & primers from some Illumina kits, side by side with the Illumina kit on the same sample of genomic DNA and it worked just as well as the Illumina. The cost is $50/library prep (plus costs for adapters & primers) when purchasing the 10 prep kit or $40/library when purchasing the 50 prep kit. I doubt you could source the individual components much cheaper.

    Reportedly NEB is planning on releasing kits for 454 library prep soon. Again, I imagine you will need to supply your own adapters and primers but the cost for these is marginal from some place like IDT.
    Last edited by kmcarr; 07-07-2009, 01:33 PM.

    Comment


    • #3
      Thanks for your reply. Yes, I will order own adapters and primers.
      Any idea where to order the immobilization beads?

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Advanced Methods for the Detection of Infectious Disease
        by seqadmin




        The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
        ...
        11-27-2023, 01:15 PM
      • seqadmin
        Strategies for Investigating the Microbiome
        by seqadmin




        Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...
        11-09-2023, 07:02 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 08:26 AM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 08:12 AM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 11-27-2023, 08:12 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 11-22-2023, 09:29 AM
      1 response
      60 views
      0 likes
      Last Post VilliamPast  
      Working...
      X