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  • Automated AMPure Purification

    Hello again!
    Does anyone have any experience doing the AMPureXP purification of amplicons on a robot? Specifically, we have a Biomek FX with standard CleanSEQ and AMPure protocols. These don't match the cleanup protocol within Roche's amplicon exactly, but I'll be doing a second round of purification for better size selection. Anyway, I want to use the automated protocol to purify 96-well plates, and then use the Roche recommended protocol for size selection. Does anybody doing that?
    Thanks again!
    Anthony

  • #2
    You might find some useful information here.

    Lennon N, Lintner R, Anderson S, Alvarez P, Barry A, Brockman W et al. (2010). A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454. Genome Biol 11: R15.

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    Phillip

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