Hi all, I am hoping for a lot of feedback. We don't have a sales rep or application scientist working our region, so I could really use updates on how well the FLX+ upgrades are working with amplicons. Are these supported yet? Are the data better than with the standard FLX runs? Appreciate it.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
There was some 'mis-communication' about whether the V2.8 upgrade of the Software Pipeline would support Amplicon sequencing at the FLX+ (~700nts-800nts). [The 'Shotgun' mode is for completeness and works quite nicely - when the rest of the reagents work; another separate problem we have had]
I'm sure the people I was talking to at Roche back in May (2012) said that the pipeline would and "be available Q3 2012". So we waited for the upgrade and got ourselves nominated as a test site for it to try it out.
Then when I asked at the start of this month as an update on the release date...no, it was just an instrument control upgrade (mostly) and FLX+ for Amplicons remains unsupported.
So, yes I had misunderstood the contents of the telephone conversation I had with Roche Service in May. I'm very disinclined to believe this actually and more likely given my notes there was miscommunication inside Roche. Whatever.
The ultimate result is the same: FLX+ for Amplicons is unsupported, as per the instrument specifications on the Roche WWW Site.
We for this to change and were thanked politely by Roche for our feedback but no commitment was made etc.
Comment
-
No, not for general release. Asking our wet lab technician I'm told our system is getting upgraded....when our FAS next passes by (usually once a week, but time dependent on our respective other tasks).
We have heard that V 2.8 does offer longer reads but this relates to minor, incremental improvement (I speculate better default parameters resulting in less trimming of the read) rather than full support of the Amplicon at 700nt / FLX+ lengths as discussed in this thread.
Version 2.7 is the latest described on the my454 WWW site with a "Analysis Software Tutorial" so I presume that is the current release. If you want inquire at: [email protected] to Roche directly.
Comment
-
I'm not sure when v2.8 will be out, but it should be soonish maybe?? I've been told it should improve obtaining longer shotgun reads as there'll be a new reagent flow introduced for XL+ kits based on 8 flows/cycle instead of the standard 4 they've used for ages. I don't think it'll support amplicons though.
Comment
Latest Articles
Collapse
-
by seqadmin
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...-
Channel: Articles
12-02-2024, 01:49 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Today, 08:24 AM
|
0 responses
5 views
0 likes
|
Last Post
by seqadmin
Today, 08:24 AM
|
||
Started by seqadmin, Yesterday, 07:41 AM
|
0 responses
6 views
0 likes
|
Last Post
by seqadmin
Yesterday, 07:41 AM
|
||
Started by seqadmin, 12-11-2024, 07:45 AM
|
0 responses
11 views
0 likes
|
Last Post
by seqadmin
12-11-2024, 07:45 AM
|
||
Started by seqadmin, 12-10-2024, 07:59 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
12-10-2024, 07:59 AM
|
Comment