Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • using Mothur to demultiplex plate prepared using RLMIDs

    Hi,
    I am trying to switch over from the Roche tools (sfffile, sffinfo..) to using Mothur to demultiplex some metagenomics data. However one plate was done using RLMIDs
    I am not sure as to how to set up the oligo file in such a case to process the data.
    There seems to be two barcode sequences perline as opossed to a single barcode
    in the MIDconfig.parse file:

    mid = "RL1", "ACACGACGACT", 2, "AGTCGTGGTGT";
    ....

    I am wondering what the two barcodes represent and whether it is possible to set up
    Mothur to process this plate.

    Thanks!

  • #2
    I don't know about Mothur, but RLMIDs are Y adaptors so the two sequences are the MID sequence on both strands of the adaptor.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Quality Control Essentials for Next-Generation Sequencing Workflows
      by seqadmin




      Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.

      Nucleic Acid Quality Control
      Preparing for NGS starts with isolating the...
      02-10-2025, 01:58 PM
    • seqadmin
      An Introduction to the Technologies Transforming Precision Medicine
      by seqadmin


      In recent years, precision medicine has become a major focus for researchers and healthcare professionals. This approach offers personalized treatment and wellness plans by utilizing insights from each person's unique biology and lifestyle to deliver more effective care. Its advancement relies on innovative technologies that enable a deeper understanding of individual variability. In a joint documentary with our colleagues at Biocompare, we examined the foundational principles of precision...
      01-27-2025, 07:46 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 02-07-2025, 09:30 AM
    0 responses
    65 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-05-2025, 10:34 AM
    0 responses
    100 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-03-2025, 09:07 AM
    0 responses
    79 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 01-31-2025, 08:31 AM
    0 responses
    44 views
    0 likes
    Last Post seqadmin  
    Working...
    X