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Difference between 454 pair end library and 454 standard library

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  • maubp
    replied
    In terms of the SFF files themselves (as far as I know) there is no difference at all. There is nothing in the file format or the Roche XML meta data block that I have seen to tell you if the data is single reads or paired ends.

    It is all down to the sample preparation. The Roche 454 website has a nice summary of how this works:
    http://www.454.com/products-solution...-end-reads.asp

    If you know you have a paired end SFF file, you should be able to find the linker sequences in the reads - see:
    http://seqanswers.com/forums/showthread.php?t=2698

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  • Difference between 454 pair end library and 454 standard library

    Hi,
    Can anybody list out the main difference between the 454 pair end library and 454 standard library?
    Thanks a lot
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