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  • mastal
    replied
    RReassembling transcriptome using recently published genome

    The sff files should have all the info you need - you can extract the fasta and qual values from the sff file.

    The format to use will depend on the assembler you choose, and what formats it accepts as input.

    Leave a comment:


  • Reassembling transcriptome using recently published genome

    Hi all,

    I have 454 Roche transcriptome assembly results. It is a single transcriptome. It is assembled "de novo". Much recently a very closely related genome was published. So I want to reassemble with this new genome as "reference". I have 2 folders in the output file given to us by the company, they are 'sff' and "assembly". I cannot find a fastq file. Do I really need fastq file for assembling? I have .qual, .fna &. ace files. Are these files along with "new genome" enough for re-assembly ?

    thank u
    raghul

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