I did 15 runs of 16s rDNA Amplicon in passed few days,
and 2.5 run within 16s rDNA region V1-V3,which the amplicon about 500bp long,
but there lots of short reads about 250-300bp,60-100bp,(sequence from A-end)
I selected ten short reads ,and blast to NCBI show that all match the 16s rDNA except the 3‘-end,about 10-15 bases,which seems the sequence of the primer B .
I wonder that does the primer B will generate non-specific amplification between 16s rDNA region v1-v3 ?
and 2.5 run within 16s rDNA region V1-V3,which the amplicon about 500bp long,
but there lots of short reads about 250-300bp,60-100bp,(sequence from A-end)
I selected ten short reads ,and blast to NCBI show that all match the 16s rDNA except the 3‘-end,about 10-15 bases,which seems the sequence of the primer B .
I wonder that does the primer B will generate non-specific amplification between 16s rDNA region v1-v3 ?
Comment