Hi LMcSeq,
Although you can use the 500bp setting suggested in our protocol (http://covarisinc.com/pdf/pn_400056.pdf), the settings in the attached file works quite well with higher concentrations of DNA up to 50ug in 120ul total sample volume. The setting generate an average size range of 425-475bp, and should be better suited with starting material length of less than 10kb.
Thank you
hamid
-
Hello,
Just to make sure I provide you the correct information, is your intention to use a hydroshear to shear down to 8kb, and then use the Covaris instrument to shear down to 500bp?
Thank you
hamidLeave a comment:
-
Roche 454 Paired End with Covaris Shearing
Hi all,
I'm working on the paired end process using the Roche/454 system for 8kb frags. I'm using the hydroshear for the initial fragmentation, but intend to use the Covaris for the downstream fragmentation step to eliminate the nebulization process.
Does anyone know if there are any adjustments required to the Covaris shearing program (we use the recommended settings for 500bp)?
Thanks!
-
Developments in sequencing technologies and methodologies have transformed the field of epigenetics, giving researchers a better way to understand the complex world of gene regulation and heritable modifications. This article explores some of the diverse sequencing methods employed in the study of epigenetics, ranging from classic techniques to cutting-edge innovations while providing a brief overview of their processes, applications, and advances.
Methylation Detect...05-31-2023, 10:46 AM -
Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...05-23-2023, 12:26 PM -
Continuing from our previous article, we share variant analysis and genome assembly tools recommended by our experts Dr. Medhat Mahmoud, Postdoctoral Research Fellow at Baylor College of Medicine, and Dr. Ming "Tommy" Tang, Director of Computational Biology at Immunitas and author of From Cell Line to Command Line.
Variant detection and analysis tools
Mahmoud classifies variant detection work into two main groups: short variants (<50...05-19-2023, 10:03 AM
Leave a comment: