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  • No key wells

    Hi everyone!

    could anybody explain me what a "no key" in the GS Run Browser application’s Wells tab means?, because we are having a lot of these wells.

    thank you

    Isabel

  • #2
    Those are wells where no bead was detected- control or library.

    Comment


    • #3
      key is a 4nt sequence on 454 adaptor/primer which is next to sequencing primer site.

      I think no key means the beads didn't carry the right DNA template. Did you mix 2 different DNA libraries (amplicon and rapid library) in the same region? If that's not the case, you may want to send a run report to tech support to check if there is any fluidic issue with the machine.

      Comment


      • #4
        Dear Newmoon,
        we didn't mix experiments, and when we sent the report to the technical service, they only told us that it could be a warning with the enzime chiller temperature plot, but we don't think that this was the reason.

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