Hi there
Can anyone help me with this conundrum? Roche state in a flyer that you should be able to sequence E. coli (~4mb) using only the 8kb paired-end protocol to 20x coverage on a 1/4 plate, suggesting you can get 320mb of data from an entire run/picotiterplate.
However, the table in Roche technical bulletin TCB-09008 seems to suggest you only get 100mb per run when using the 8kb-library.
Can anyone guide as to the reason for the discrepancy?
Cheers
Nick.
Can anyone help me with this conundrum? Roche state in a flyer that you should be able to sequence E. coli (~4mb) using only the 8kb paired-end protocol to 20x coverage on a 1/4 plate, suggesting you can get 320mb of data from an entire run/picotiterplate.
However, the table in Roche technical bulletin TCB-09008 seems to suggest you only get 100mb per run when using the 8kb-library.
Can anyone guide as to the reason for the discrepancy?
Cheers
Nick.
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