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I would like to sequence 16s from more than 400 sample of nasopharyngeal samples by pyrosequencing.
It seems to be a good method to do that saving time and resources. Does anyone have any experience in this? Someone with a direct experience in issues as sample preparation, quality control etc
We want to use 27F-534R primers to sequencing V1-V3 region. I had thought to add them adaptor A and B, and then a Broad Institute bardcode. I am not sure if I need one bardcode per sample, and therefore I would needed >400 bardcodes between 7-12 pb. I suposed a linker is neccesary in all design primers.
It seems to be a good method to do that saving time and resources. Does anyone have any experience in this? Someone with a direct experience in issues as sample preparation, quality control etc
We want to use 27F-534R primers to sequencing V1-V3 region. I had thought to add them adaptor A and B, and then a Broad Institute bardcode. I am not sure if I need one bardcode per sample, and therefore I would needed >400 bardcodes between 7-12 pb. I suposed a linker is neccesary in all design primers.
