I would like to sequence 16s from more than 400 sample of nasopharyngeal samples by pyrosequencing.
It seems to be a good method to do that saving time and resources. Does anyone have any experience in this? Someone with a direct experience in issues as sample preparation, quality control etc
We want to use 27F-534R primers to sequencing V1-V3 region. I had thought to add them adaptor A and B, and then a Broad Institute bardcode. I am not sure if I need one bardcode per sample, and therefore I would needed >400 bardcodes between 7-12 pb. I suposed a linker is neccesary in all design primers.
-
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...09-23-2024, 06:35 AM -
During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.
Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...09-09-2024, 10:59 AM