Hi SEQcommunity,
we're starting a 454 project to look for mutations/SNPs in several amplicons of a particular gene. We're not sure about the coverage needed. Roche told us we need a covergae of 1000 to detect a variant with 5% frequency. Does anyone got experience with this issue?
Thanks in advance
we're starting a 454 project to look for mutations/SNPs in several amplicons of a particular gene. We're not sure about the coverage needed. Roche told us we need a covergae of 1000 to detect a variant with 5% frequency. Does anyone got experience with this issue?
Thanks in advance