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  • pmiguel
    replied
    Actually, with the results I saw from recent v3 runs I was wondering if anyone had pushed out past 100 nt on HiSeq chemistry already?

    --
    Phillip

    Leave a comment:


  • scrosby
    replied
    Forgive me...

    I should have said 'soon' going to to 150 PE. Before the end of the year, I believe?

    Leave a comment:


  • pmiguel
    replied
    Originally posted by scrosby View Post
    With Illumina now going to 300bp
    link?

    --
    Phillip

    Leave a comment:


  • scrosby
    replied
    With Illumina now going to 300bp, does there remain a viable role for 454?

    Leave a comment:


  • gstitan
    replied
    Cangs

    Does anybody already have to use CANGS? If yes he could explain to me why it filters primers sequences while this filter is already made by 454?

    Leave a comment:


  • edge
    replied
    Hi,

    You have already summary out the tech of Roche's 454 GS20 / FLX / Titanium?
    I interesting about it too

    Originally posted by ECO View Post
    There isn't anything here yet!

    If you are interested in writing a tech summary, just post it below and I will rearrange the thread as appropriate.

    Alternatively if anyone wants to post tech sheets, manuals, etc, I will write it. I just don't know enough of it beyond "pyrosequencing, picotiter plates, and a ton of beads".

    Leave a comment:


  • SeqWhiskey
    replied
    SV

    Look out for the SV prep on titanium. There is a pretty serious typo under the DNA capture step. 3.2.7 the volume to evenly distribute capture beads is off. It says 40ul per rxn, it's really more like 70+ul. In fact completely ignore this section and prep each rxn in individual 1.7ml tubes. The roche tech agrees with me... just wash with 3-400ul of cap bead wash buff so you don't run out.... Then again, does Ti even work as advertised?

    Leave a comment:


  • ECO
    replied
    Originally posted by bioinfosm View Post
    I am confused by this .. "pyrosequencing from illumina" !
    Originally posted by monad View Post
    Confused here too.
    Illmunia currently produces 15-20GB from single run of paired-end already.
    Protocol will be available sometime early next year.
    They bought Avantome.

    Leave a comment:


  • monad
    replied
    Originally posted by bioinfosm View Post
    I am confused by this .. "pyrosequencing from illumina" !
    Confused here too.
    Illmunia currently produces 15-20GB from single run of paired-end already.
    Protocol will be available sometime early next year.

    Leave a comment:


  • bioinfosm
    replied
    I am confused by this .. "pyrosequencing from illumina" !

    Leave a comment:


  • Tom Bair
    replied
    quick summary of titanium from 454 site

    Needed this for something else may be incomplete or wrong but ..

    400-600 million high-quality, filter-passed bases per run
    10 hours
    average length 400 bp
    more than 1x10^6 reads

    a cluster from roche is recommended for signal processing from www.pssclabs.com. It looks like image processing will still happen on the GS-FLX and 9G of cwf (wells) files need to go the the cluster

    from what I can tell it is a 5 node system (20 cores) the only specs I can find say redhat with either openMPI or MPI2 (mpiexec), SunGrid as scheduler

    runs will be 35GB (26GB images)

    A test data-set with a configuration tester is promised soon

    Rumor has it that bead breaking will be via centrifuge in the new protocol

    Leave a comment:


  • ECO
    started a topic Tech Summary: Roche's 454 GS20 / FLX / Titanium

    Tech Summary: Roche's 454 GS20 / FLX / Titanium

    There isn't anything here yet!

    If you are interested in writing a tech summary, just post it below and I will rearrange the thread as appropriate.

    Alternatively if anyone wants to post tech sheets, manuals, etc, I will write it. I just don't know enough of it beyond "pyrosequencing, picotiter plates, and a ton of beads".

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