Actually, with the results I saw from recent v3 runs I was wondering if anyone had pushed out past 100 nt on HiSeq chemistry already?
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Phillip
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Forgive me...
I should have said 'soon' going to to 150 PE. Before the end of the year, I believe?
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With Illumina now going to 300bp, does there remain a viable role for 454?
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Cangs
Does anybody already have to use CANGS? If yes he could explain to me why it filters primers sequences while this filter is already made by 454?
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Hi,
You have already summary out the tech of Roche's 454 GS20 / FLX / Titanium?
I interesting about it too
Originally posted by ECO View PostThere isn't anything here yet!
If you are interested in writing a tech summary, just post it below and I will rearrange the thread as appropriate.
Alternatively if anyone wants to post tech sheets, manuals, etc, I will write it. I just don't know enough of it beyond "pyrosequencing, picotiter plates, and a ton of beads".
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SV
Look out for the SV prep on titanium. There is a pretty serious typo under the DNA capture step. 3.2.7 the volume to evenly distribute capture beads is off. It says 40ul per rxn, it's really more like 70+ul. In fact completely ignore this section and prep each rxn in individual 1.7ml tubes. The roche tech agrees with me... just wash with 3-400ul of cap bead wash buff so you don't run out.... Then again, does Ti even work as advertised?
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Originally posted by bioinfosm View PostI am confused by this .. "pyrosequencing from illumina" !Originally posted by monad View PostConfused here too.
Illmunia currently produces 15-20GB from single run of paired-end already.
Protocol will be available sometime early next year.
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quick summary of titanium from 454 site
Needed this for something else may be incomplete or wrong but ..
400-600 million high-quality, filter-passed bases per run
10 hours
average length 400 bp
more than 1x10^6 reads
a cluster from roche is recommended for signal processing from www.pssclabs.com. It looks like image processing will still happen on the GS-FLX and 9G of cwf (wells) files need to go the the cluster
from what I can tell it is a 5 node system (20 cores) the only specs I can find say redhat with either openMPI or MPI2 (mpiexec), SunGrid as scheduler
runs will be 35GB (26GB images)
A test data-set with a configuration tester is promised soon
Rumor has it that bead breaking will be via centrifuge in the new protocol
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Tech Summary: Roche's 454 GS20 / FLX / Titanium
There isn't anything here yet!
If you are interested in writing a tech summary, just post it below and I will rearrange the thread as appropriate.
Alternatively if anyone wants to post tech sheets, manuals, etc, I will write it. I just don't know enough of it beyond "pyrosequencing, picotiter plates, and a ton of beads".Tags: None
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