Actually, with the results I saw from recent v3 runs I was wondering if anyone had pushed out past 100 nt on HiSeq chemistry already?
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Phillip
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Forgive me...
I should have said 'soon' going to to 150 PE. Before the end of the year, I believe?Leave a comment:
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With Illumina now going to 300bp, does there remain a viable role for 454?Leave a comment:
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Cangs
Does anybody already have to use CANGS? If yes he could explain to me why it filters primers sequences while this filter is already made by 454?
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SV
Look out for the SV prep on titanium. There is a pretty serious typo under the DNA capture step. 3.2.7 the volume to evenly distribute capture beads is off. It says 40ul per rxn, it's really more like 70+ul. In fact completely ignore this section and prep each rxn in individual 1.7ml tubes. The roche tech agrees with me... just wash with 3-400ul of cap bead wash buff so you don't run out.... Then again, does Ti even work as advertised?Leave a comment:
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quick summary of titanium from 454 site
Needed this for something else may be incomplete or wrong but ..
400-600 million high-quality, filter-passed bases per run
10 hours
average length 400 bp
more than 1x10^6 reads
a cluster from roche is recommended for signal processing from www.pssclabs.com. It looks like image processing will still happen on the GS-FLX and 9G of cwf (wells) files need to go the the cluster
from what I can tell it is a 5 node system (20 cores) the only specs I can find say redhat with either openMPI or MPI2 (mpiexec), SunGrid as scheduler
runs will be 35GB (26GB images)
A test data-set with a configuration tester is promised soon
Rumor has it that bead breaking will be via centrifuge in the new protocolLeave a comment:
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Tech Summary: Roche's 454 GS20 / FLX / Titanium
There isn't anything here yet!
If you are interested in writing a tech summary, just post it below and I will rearrange the thread as appropriate.
Alternatively if anyone wants to post tech sheets, manuals, etc, I will write it. I just don't know enough of it beyond "pyrosequencing, picotiter plates, and a ton of beads".
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The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
...Yesterday, 01:15 PM -
Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...11-09-2023, 07:02 AM
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