Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • running amplicons together with non amplicon lanes

    Hi,
    I wanted to know whether is is ok to use one plate in which some regions are for amplicons and others are not. If so, what kind of run should it be? Image processing only ?

  • #2
    Hi Dina,

    If the amplicons are in a separate region of the plate from the shotgun libraries, it's fine. You'll have to do the signal processing twice, once for each library type.

    Hope this helps.

    Comment


    • #3
      There were instructions on the my454 site http://454.com/downloads/my454/docum...Experiment.pdf for creating a new choice in the 454 run client. The new choice is:

      “Image Processing Only Amplicons”

      So I guess the question becomes, if one is doing a run with regions containing amplicons and other regions containing non-amplicons, should one choose “Image Processing Only Amplicons” or “Image Processing Only”.

      Probably doesn't matter, since the console server will only do image processing either way, what happens past that point is up to scripts running on whatever cluster is used to do the downstream processing.

      --
      Phillip

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Best Practices for Single-Cell Sequencing Analysis
        by seqadmin



        While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
        06-06-2024, 07:15 AM
      • seqadmin
        Latest Developments in Precision Medicine
        by seqadmin



        Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

        Somatic Genomics
        “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
        05-24-2024, 01:16 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 08:58 AM
      0 responses
      8 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 02:20 PM
      0 responses
      14 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 06-07-2024, 06:58 AM
      0 responses
      181 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 06-06-2024, 08:18 AM
      0 responses
      231 views
      0 likes
      Last Post seqadmin  
      Working...
      X