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sequencing reads simulation

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  • sequencing reads simulation

    Hi,



    Can anyone explain the results of wgsim of me? What does the character 'R', 'M', 'Y' stands for in the third column? What is the meaning of the second column? In addition, if I have two haplotypes of a individual (with konwn heterozygous and homozygous variants), how can simulate the sequencing reads of this individual?



    Thanks




    20 62504 A R +

    20 64077 A R +

    20 64771 A M +

    20 67086 C Y +

    20 67502 G C -

    20 68336 T G -

    20 68678 A R +

    20 70639 T Y +

    20 70788 T W +

    20 73011 T Y +

    20 75088 T W +

    20 76026 T W +

    20 76306 C Y +

  • #2
    They are IUPAC codes, and most likely heterozygous variants.

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