100bp paired-end RNA-seq library

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miu007

Junior Member

Join Date: Jan 2013

Posts: 4
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#1

100bp paired-end RNA-seq library

02-28-2013, 10:31 AM

Hi folks,

Does anyone have experience making libraries for 100bp paired-end sequencing? What is the optimal insert size? How to generate fragments suitable for this purpose?Thanks.
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luc

Senior Member

Join Date: Dec 2010

Posts: 469
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#2

02-28-2013, 02:10 PM

These two protocols are very good:

High-Throughput Illumina Strand-Specific RNA Sequencing Library Preparation.
Cold Spring Harb Protoc. 2011;2011(8)
Authors: Zhong S, Joung JG, Zheng Y, Chen YR, Liu B, Shao Y, Xiang JZ, Fei Z, Giovannoni JJ

A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq
Lin Wang, Yaqing Si, Lauren K. Dedow, et al. 2011 PLoS ONE 6(10)
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