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Help to plan experiment

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  • Help to plan experiment

    Hello everyone,

    I am a biology student and I need to check the microbial population in the intestine before and after taking a certain medication, to see if the drug affects the microbe abundance in the intestine... I need to plan this experiment using NGS and I am LOST!
    please can someone help me to plan this experiment...? what NGS technology should I use after extraction of DNA/RNA samples?

    Thank You!

  • #2
    Have you looked into 16s rDNA sequencing? It may not be the best solution, but there are lots of papers out there using this method.


    • #3
      1. Think very hard what biological questions you want to address
      2. Find out how much funding you have
      3. Read a ton of papers
      4. Make an informed decision