Help to plan experiment

Nullie

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Join Date: Jun 2013

Posts: 9
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#1

Help to plan experiment

06-02-2013, 12:53 AM

Hello everyone,

I am a biology student and I need to check the microbial population in the intestine before and after taking a certain medication, to see if the drug affects the microbe abundance in the intestine... I need to plan this experiment using NGS and I am LOST!
please can someone help me to plan this experiment...? what NGS technology should I use after extraction of DNA/RNA samples?

Thank You!
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microgirl123

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Join Date: Jun 2012

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#2

06-03-2013, 04:47 AM

Have you looked into 16s rDNA sequencing? It may not be the best solution, but there are lots of papers out there using this method.
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rhinoceros

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Join Date: Apr 2013

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#3

06-03-2013, 05:36 AM

1. Think very hard what biological questions you want to address
2. Find out how much funding you have
3. Read a ton of papers
4. Make an informed decision

savetherhino.org
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