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Thanks! I will look into that. But you're still not getting alignment info, are you?Leave a comment:
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Are you using pipeline v1.5.1? if so after you run eland you can run the split_on_index.py script and it will bin your results by barcode.
The script needs an .xml sheet that tells it what barcodes to look for in each lane. The usage text for the script will tell you the format of the xml file expected.Leave a comment:
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barcode analysis
Hello:
Does anyone have a clever (ie, one step) way of doing ELAND alignments on reads with 5' index sequences while maintaining the index sequence info for each individual read? The only ways I've heard about involve re-analyzing, either from images using a custom runinfo.xml file, or splitting the reads by index sequence then re-aligning with those sequences removed. I'm too lazy for that!
CN
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At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...09-26-2023, 06:26 AM -
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM
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