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Hello,
I would like to sequence 250 bases on `Illumina Novaseq S4`, on read 2.
1) Kits of illumina come in the following sizes: https://emea.illumina.com/systems/se...fications.html
2) You can setup the sequencer for asymmetric sequencing, so that from a standard paired-end 2*100bp kit reads `150` bases from R1, and only `50` of the R2 (or vice versa).
3) On `SP` kit they now support `2 x 250 bp`, but not on `S4`.
4) On the (largest) `S4`, the longest kit is `2*150`. It would be theoretically possible to sequence `250 + 50`. Or if that's too much, `200 + 100` is okay too.
The read quality at 150 bases is excellent, so I expect at least 200 useful bases.
- Did anyone tried this, or could point me to relevant information?
- I read q82817 and q59603
- Can you even specify such strong asymmetry in the software?
- Very importantly: Are there any risk of losing the lane?
- Are there any other risks (other than quality issues beyond 150 bases, which i assume I can easily trim away)?
Thanks so much for your time!
ps. originally posted on biology.stackexchange-112881, but it might be the wrong audience.
I would like to sequence 250 bases on `Illumina Novaseq S4`, on read 2.
1) Kits of illumina come in the following sizes: https://emea.illumina.com/systems/se...fications.html
2) You can setup the sequencer for asymmetric sequencing, so that from a standard paired-end 2*100bp kit reads `150` bases from R1, and only `50` of the R2 (or vice versa).
3) On `SP` kit they now support `2 x 250 bp`, but not on `S4`.
4) On the (largest) `S4`, the longest kit is `2*150`. It would be theoretically possible to sequence `250 + 50`. Or if that's too much, `200 + 100` is okay too.
The read quality at 150 bases is excellent, so I expect at least 200 useful bases.
- Did anyone tried this, or could point me to relevant information?
- I read q82817 and q59603
- Can you even specify such strong asymmetry in the software?
- Very importantly: Are there any risk of losing the lane?
- Are there any other risks (other than quality issues beyond 150 bases, which i assume I can easily trim away)?
Thanks so much for your time!
ps. originally posted on biology.stackexchange-112881, but it might be the wrong audience.
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