This may help you. Go to this page:
and click on "MiSeq: Does My Run Look Good?"
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High percentage Q30 or higher bases
High amount of data
High percentage reads that reach full length
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A ok run is when its within manufacturer specs both output and base quality wise. A good run is slightly better. And an excellent run is when no one complains about data afterwards.
Our best so far was ~360GB off a HiSeq cell 2x101+index.
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What is a good run? Do you have a QC to determine that.
Alright, I was in a different thread and at one point the discussion went slightly of topic but it was clear to me that everyone had a different opinion on what is a good MiSeq/HiSeq run. So in a Good Mythical Morning sense "let's talk bout that".
In your own opinion what makes a good run? Also do you have a specific QC to determine that.Tags: None
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by seqadmin
At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...-
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09-26-2023, 06:26 AM -
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by seqadmin
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...-
Channel: Articles
09-07-2023, 11:15 PM -
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