Thanks peromhc!
I will just send this sample for sequencing.
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I just did a RNA prep using NEB reagents and QIAGEN spin columns using ~900ng total RNA and it was fine.. Your 5ug will be perfect.Leave a comment:
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Thanks shurjo!
They are total RNA sample. Because in the protocol, 1-2 ug was used as the starting material. But the people in core facility told me it is better give them more, out last sample for sequencing was around 7 ug. Therefore, just ask experienced RNA-Sequencers if 5 ug is fine.
Nice weekend!
WeiLeave a comment:
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5 microgram for Illumina
Dear All,
I have only 5 microgram in my sample, is that enough for Illumina sequencing?
Thanks a lot!
Wei
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