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  • luc
    replied
    The Illumina protocol will more convenient in the long run. If one can avoid ligations and the other library prep steps, I would suggest to do so.

    Leave a comment:


  • floresu
    started a topic 16S library prep + Nebnext

    16S library prep + Nebnext

    I want to characterize some microbial communities by Miseq sequencing of 16S libraries using the primers 341F/805R (reported in Klindworth 2012). Illumina's "16S Metagenomic Sequencing Library Preparation" uses the same primers but modified to include overhang sequences. Currently in the lab we have the NebNext DNA Library Prep Kit.

    What would be the difference of preparing the libraries (using NebNext) with PCR products with and without overhang seqs?

    Does it affect overall analysis sensitivity?

    I would appreciate any advice you guys could give me.

    Thanks in advance!

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

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