-
1.8x volume should be good enough to get rid of <100bp fragments. 1x volume is good for <200bp. I have heard of different lots of SPRI beads giving different break points so YMMV. It shouldn't take any time to try out different volumes on some 100bp ladder, which is what we do.Leave a comment:
-
SPRI bead for PCR cleanup in illumina pair end library preparation
Hi everybody,
I am preparing 300bp and 600bp illumina pair end library. Can anybody please let me know which SPRI beads and in what concentration should I use for cleaning up the PCR product in the end step. Thanks for the help.
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Leave a comment: