Header Leaderboard Ad


Consistent low MiSeq cluster density and PhiX alignment with high quality reads (PF%)



No announcement yet.
  • Filter
  • Time
  • Show
Clear All
new posts

  • #16
    Originally posted by thermophile View Post
    is your cluster density the same top and bottom of the flow cell? if you have sig more reads on the bottom you may be carrying over etoh from the ampure cleanup
    Hi thermophile, I'm having similar issues with my MiSeq runs and noticed your comment. How would this effect my run? I can't find any other information about this.

    EDIT: Just seen your reply my apologies!

    Best wishes
    Last edited by razzaowl; 01-05-2022, 09:52 AM.


    Latest Articles


    • seqadmin
      A Brief Overview and Common Challenges in Single-cell Sequencing Analysis
      by seqadmin

      ​​​​​​The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...

      01-24-2023, 01:19 PM
    • seqadmin
      Introduction to Single-Cell Sequencing
      by seqadmin
      Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

      The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes
      01-09-2023, 03:10 PM