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Consistent low MiSeq cluster density and PhiX alignment with high quality reads (PF%)

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  • #16
    Originally posted by thermophile View Post
    is your cluster density the same top and bottom of the flow cell? if you have sig more reads on the bottom you may be carrying over etoh from the ampure cleanup
    Hi thermophile, I'm having similar issues with my MiSeq runs and noticed your comment. How would this effect my run? I can't find any other information about this.

    EDIT: Just seen your reply my apologies!

    Best wishes
    R
    Last edited by razzaowl; 01-05-2022, 09:52 AM.

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