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  • How to enter Unique Dual Index Primer into BaseSpace

    I'm using unique dual index primer pairs for my experiments. I'm multiplexing around 12 samples per NextSeq run. I don't know how to create a custom library in BaseSpace that will allow me to assign the unique primer pairs to all my samples as it's limited by a 12 x 8 format (12- i7 indexes and 8-i5 indexes, I need to be able to enter 12 i5 indexes). Any help would be greatly appreciated.

  • #2
    I ran into a similar issue recently. If you have the Dx version of the NextSeq, there is a software update that can do FASTQ generation on the machine similar to the MiSeq.

    The other option is a work around where you will have to create two custom library kit. Each kit can do 8 UDI samples if you set your samples in a diagonal so that each row and column will have an unique index. After that just pool them together in the pool tab in BaseSpace.

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    • #3
      I don't have the Dx version but your workaround will be just fine, I didn't think it would let me combine samples from two different "kits". Thanks for the help!

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      • #4
        custom library

        https://support.illumina.com/bulleti...sequence-.html

        Here's the bulletin from Illumina describing how to create a custom library prep kit in BaseSpace.

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