Originally posted by SNPsaurus
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In relation to this observation, I recently started a run that ended with a Qscore of 78% and a Read PF of 96%. It had a total read of 4.9M. Its underclustered as a result of using the Qubit value for loading rather than using the qPCR value and also we multiplexed 350 samples (amplicon). However, one surprising event is that half of the samples are little or no reads while the other indexed samples have the 4.9m reads generated. Any explanation for this.
Thanks
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