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What happens try to amp/lin/block/hyb paired end libraries on a single read flowcell?

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  • What happens try to amp/lin/block/hyb paired end libraries on a single read flowcell?

    Are the adapters the same or will this make a difference. Thanks

  • #2
    Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.

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    • #3
      Originally posted by hoisinjl View Post
      Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.
      What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?

      It looks to me like the adapter sequences are different, and that it wouldn't work the other way around.

      Any thoughts?

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      • #4
        Originally posted by gro-seeker View Post
        What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?
        No, libraries prepared with single read kits are not compatible with paired end cluster generation kits and flowcells.

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        • #5
          All right, thanks so much! We'll have to go with single reads then.

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