What happens try to amp/lin/block/hyb paired end libraries on a single read flowcell?

[Dr. Hybe]

Are the adapters the same or will this make a difference. Thanks

[hoisinjl]

Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.

[gro-seeker]

Libraries that are prepared as paired-end libraries, will work on single-read flowcells without a problem.

What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?

It looks to me like the adapter sequences are different, and that it wouldn't work the other way around.

Any thoughts?

[kmcarr]

What about the other way around? Could libraries prepared as single-read libraries (eg. with the small RNA sequencing adapters & primers) be run on paired-end flowcells?

No, libraries prepared with single read kits are not compatible with paired end cluster generation kits and flowcells.

[gro-seeker]

All right, thanks so much! We'll have to go with single reads then.