Any luck?
Did you have any luck at 1/5x library construction? Can you post the source of the original protocol you used?
-
1/5 Nextera Flex number of cycles
Hi,
We are about to start a fairly large experiment with the Nextera Flex kit in a very limited time. After some testing and reading experiences we have decided to do the kit at 1/5 strength. Now we are wondering if the DNA input should be adjusted as well. We will use 6uL DNA input (1/5 of 30), but does this mean that should contain >20ng (1/5 of 100) or still >100 to reach normalization with 5 cycles? Do you add a couple of cycles and if so how many? Is there such a thing as too many cycles? (we've been advised to do 16 cycles but that sounds like way too many but what about 8 or 10?).
I'm confident we will have enough library for sequencing but because of the number of samples that we plan to pool I am really hoping to use the kit's normalization and to avoid normalization by dilution.
-
At the intersection of cytogenetics and genomics lies the exciting field of cytogenomics. It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. By integrating cytogenetic techniques with genomic analysis, researchers can effectively investigate chromosomal abnormalities related to diseases, particularly...09-26-2023, 06:26 AM -
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM
Leave a comment: